Effect of culture time on secondary metabolite production from MeJA-stimulated in vitro roots of Hyoscyamus niger L.

This study was conducted to determine the effects of different culture times on root growth and accumulation secondary metabolites in adventitious roots from Hyoscyamus niger petioles. For this purpose, transferred media containing 1 mM methyl jasmonate (MeJA) were harvested at 11 times, starting day 30. After harvest, fresh dry weights index as parameters growth, well amounts tropane alkaloids phenolic compounds, examined. It found that between 12th 30th highest. The highest scopolamine obtained days 15, 18, 21. hyoscyamine 12 15. total content 19.33 mg g-1 cultures HPLC analyses revealed ferulic acid, caffeic o-coumaric p-coumaric rutin, quercetin not detected roots. On other hand, gallic catechin, epicatechin, vanillin, cinnamic rosmarinic chlorogenic acid showed significant changes depending times. Considering all results, it optimum time for production H. stimulated with 1mM MeJA 15 days.